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Rabbit Anti-Egr1 antibody (bs-1076R)
訂購熱線:400-901-9800
訂購郵箱:[email protected]
訂購傳真:010-58129612
技術支持:[email protected]
說明書: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
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產品編號 bs-1076R
英文名稱 Egr1
中文名稱 早期生長應答蛋白1抗體
別    名 Egr-1; Early growth response 1; Krox-24; Ngf1; Ngfi; NGFI-A; zif-268; Egr1; A530045N19Rik; egr; Egr-1; ETR103; Krox-1; Krox-24; Krox24; NGF1-A; NGFI-A; NGFIA; TIS8; Zenk; Zfp-6; AT 225; AT225; Early growth response protein 1; G0S 30; G0S30; KROX 24; Krox 24 protein; KROX24; TIS 8; TIS8; Transcription factor ETR 103; Transcription factor ETR103; Transcription factor Zif 268; Transcription factor Zif268; Zfp 6; ZIF268; Zinc finger protein 225; ZNF 225; ZNF225; EGR1_HUMAN.  
研究領域 細胞生物  免疫學  信號轉導  生長因子和激素  細胞周期蛋白  轉錄調節因子  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, 
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2ug/test IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 60kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Egr-1:401-500/453 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹 The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq].

Function:
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
P18146

Gene ID:
1958

Database links:

Entrez Gene: 1958 Human

Entrez Gene: 13653 Mouse

Omim: 128990 Human

SwissProt: P18146 Human

SwissProt: P08046 Mouse

Unigene: 326035 Human

Unigene: 708393 Human

Unigene: 181959 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Egr-1為即刻早期基因家族中的一個成員,是一種含3個鋅指結構的轉錄因子。有研究表明EGR-1在細胞生長分化中發揮重要作用,如在細胞周期G1-S期的轉換過程中Egr-1被誘導表達。
產品圖片
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Egr1 (bs-1076R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Sample:
Liver (Rat) Lysate at 40 ug
Primary: Anti- Egr1 (bs-1076R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Egr1) Polyclonal Antibody, Unconjugated (bs-1076R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Egr1 Polyclonal Antibody, Unconjugated(bs-1076R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat small intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Egr1 Polyclonal Antibody, Unconjugated(bs-1076R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Egr1 Antibody(bs-1076R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).
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