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Rabbit Anti-Caspase-12 antibody (bs-1105R)
訂購熱線:400-901-9800
訂購郵箱:[email protected]
訂購傳真:010-58129612
技術支持:[email protected]
說明書: 50ul  100ul  200ul
50ul/980.00元
100ul/1680.00元
200ul/2480.00元
大包裝/詢價

產品編號 bs-1105R
英文名稱 Caspase-12
中文名稱 半胱胺酸蛋白酶蛋白-12抗體
別    名 UNQ9415; Apoptosis related cysteine protease; CASP 12; casp12; CASP12P1; Caspase 12 pseudogene 1; CASP-12; casp12; caspase 12 (gene/pseudogene); Caspase12; CASPC_HUMAN; OTTHUMP00000207026; OTTHUMP00000207028; OTTHUMP00000207031; OTTHUMP00000207032; OTTHUMP00000219058; UNQ9415.  
Specific References  (9)     |     bs-1105R has been referenced in 9 publications.
[IF=4.784] Wang L et al. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019 Apr 15;127:18-26.   IHC-P ;  Mouse.  
[IF=2.197] Luo Q et al. Role?of the?Death?Receptor?and?Endoplasmic?Reticulum?Stress?Signaling?Pathways?in?PolyphyllinI-Regulated?Apoptosis?of?Human?Hepatocellular?Carcinoma?HepG2?Cells. Biomed Res Int. 2018 Dec 25;2018:5241941.  WB ;  Human.  
[IF=2.2] Zhang, Huiying, et al. "Glucose-regulated protein 78 may play a crucial role in promoting the pulmonary microvascular remodeling in a rat model of hepatopulmonary syndrome." Gene (2014).  IHC-P ;  Rat.  
[IF=1.88] Zhou, Yunchuan, et al. "Sulfiredoxin-1 exerts anti-apoptotic and neuroprotective effects against oxidative stress-induced injury in rat cortical astrocytes following exposure to oxygen-glucose deprivation and hydrogen peroxide."International Journal of Molecular Medicine (2015).  WB ;  Rat.  
[IF=1.89] Zhang, Lili, et al. "Increased expression of 78 kD glucose-regulated protein promotes cardiomyocyte apoptosis in a rat model of liver cirrhosis." International Journal of Clinical and Experimental Pathology 8.8 (2015): 9256-9263.  IHC-P ;  Rat.  
[IF=4.65] Yu, H., et al. "Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro."Cellular Physiology and Biochemistry 39.1 (2016): 123-136.  WB ;  Rat.  
[IF=2.29] Wang, Yu, et al. "Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways." Heart and Vessels (2016): 1-8.  WB ;  Rat.  
[IF=3.23] Wang, Yu, et al. "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes." PloS one 12.4 (2017): e0173469.  WB ;  Rat.  
[IF=3.448] Sun Y et al. Effect of endoplasmic reticulum stress-mediated excessive autophagy on apoptosis and formation of kidney stones. Life Sci. 2019 Dec 26:117232.   WB ;  Rat.  
研究領域 細胞凋亡  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse, Rat, 
產品應用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 46kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from the middle of human Caspase-12:201-300/420 
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產品介紹 Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011].

Function:
Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.

Tissue Specificity:
Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis.

Similarity:
Belongs to the peptidase C14A family.
Contains 1 CARD domain.

SWISS:
O08736

Gene ID:
100506742

Database links:

Entrez Gene: 100506742 Human

Entrez Gene: 12364 Mouse

Entrez Gene: 156117 Rat

SwissProt: Q6UXS9 Human

SwissProt: O08736 Mouse

SwissProt: Q920D5 Rat

Omim: 608633 Human

Unigene: 476989 Human

Unigene: 81078 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Caspase-12是caspase家族成員,是存在于內質網上的促凋亡因子,內質網應激早期可通過GRP(glucose-regulated protein,GRP)的表達增多來保護細胞;CASPASE-12是內質網細胞關鍵介導因子,caspase-12還是caspase-1的抑制劑。
產品圖片
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-alpha smooth muscle Actin (bs-1105R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 50 kD
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-12 (bs-1105R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45/52 kD
Observed band size: 46 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Caspase-12 (bs-1105R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (mouse fallopian tube); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (bs-1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (bs-1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Caspase-12 Polyclonal Antibody, Unconjugated(bs-1105R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat testis tissue(high fat); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(bs-R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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